Tumor Necrosis Factor-α human T0157 from MilliporeSigma

Product Specs
ItemTumor Necrosis Factor-α human
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberT0157
Concentration0.1-10 mg/mL
Molecular Weight~17.4 kDa
Specieshuman
Molecule NameHmgcr, HMGCR
NCBI Full Gene Name3-hydroxy-3-methylglutaryl-CoA reductase
NCBI Gene AliasesLDLCQ3, 3H3M
Applicationsmammalian cell culture
FormatSolution in phosphate buffered saline containing 1 mg/ml bovine serum albumin
Quantity10µG

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MilliporeSigma

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Website: www.SigmaAldrich.com/

(1) Myeloid cells interact with a subset of thyrocytes to promote their migration and follicle formation through NF-κBNat CommunDecember 6, 2023Rui-Meng Yang, Shi-Yang Song, Feng-Yao Wu, Rui-Feng Yang, Yan-Ting Shen, Ping-Hui Tu, Zheng Wang, Jun-Xiu Zhang, Feng Cheng, Guan-Qi Gao, et al.supplemented with 10% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco). TNF-α (Sigma; T0157) and lenalidomide (Selleck; S1029) were used for cell treatments. Western Blot Proteins from cell

(2) Human cortical spheroids with a high diversity of innately developing brain cell typesStem Cell Research & TherapyMarch 23, 2023Kim M. A. De Kleijn, Wieteke A. Zuure, Kirsten R. Straasheijm, Marijn B. Martens, M. Cristina Avramut, Roman I. Koning, Gerard J. M. Martensand hCSs were incubated with or without 5 ng/ml Tumor necrosis factor alpha (TNFα) (Sigma-Aldrich, T0157-10UG, dissolved in PBS) and 5 ng/ml Interleukin-1 beta (IL-1β) (Sigma-Aldrich, SRP3083-10UG, dissolved

(3) A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsiaCellular and molecular life sciences : CMLSJanuary 18, 2023Ghorbanpour SM, Richards C, Pienaar D, Sesperez K, Aboulkheyr Es H, Nikolic VN, Karadzov Orlic N, Mikovic Z, Stefanovic M, Cakic Z, et al.Cells were treated with 10 ng/mL TNF-α (as previously described [58, 60–62]; Sigma-Aldrich, USA, cat. #T0157) for 24 or 72 h with untreated cells used as a control. The cells for the control at both 24- and 72-h

(4) A Whole-Genome Sequencing Study Implicates GRAMD1B in Multiple Sclerosis SusceptibilityGenes (Basel)December 16, 2022Federica Esposito, Ana Maria Osiceanu, Melissa Sorosina, Linda Ottoboni, Bryan Bollman, Silvia Santoro, Barbara Bettegazzi, Andrea Zauli, Ferdinando Clarelli, Elisabetta Mascia, et al.for specificity of the antibody. 2.10. Reagents Stock solutions of TNFα, IFNγ, LPS (Sigma-Aldrich; T0157, I3265 and L2654, respectively), IFNβ (Rebif 44, Merck Serono, Darmstadt, Germany), or macrophage colony-stimulating

(5) Delivery of miR-654-5p via SonoVue Microbubble Ultrasound Inhibits Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells and Arterial Thrombosis and Stenosis through Targeting TCF21Oxidative Medicine and Cellular LongevityJuly 19, 2022Tao Wang, Xiaoqiang Tang, Yong Zhang, Xiaoqin Wang, Haifeng Shi, Ruohan Yin, Changjie Paninterleukin-1β (IL-1β, 40 ng/mL, MBF18, Sigma-Aldrich, USA), tumor necrosis factor-α (TNF-α, 25 ng/mL, T0157, Sigma-Aldrich, USA), platelet-derived growth factor BB (PDGF-BB, 20 ng/mL, P6101, Sigma-Aldrich, USA),

(6) DRAMâ€4 and DRAMâ€5 are compensatory regulators of autophagy and cell survival in nutrientâ€deprived conditionsThe FEBS JournalJuly 1, 2022Valentin J. A. Barthet, Michaela Mrschtik, Elzbieta Kania, David G. McEwan, Dan Croft, James O'Prey, Jaclyn S. Long, Kevin M. Ryancatalog no. 285‐IF‐100, USA), or 5 ng·mL−1 of tumor necrosis factor α (TNFα, Sigma‐Aldrich, catalog no. T0157). Tet‐on p53 Saos‐2 cells were treated with 1 µg·mL−1 of doxycycline (Sigma‐Aldrich, catalog no. D9891). Transfections

(7) Molecular Signature of Neuroinflammation Induced in Cytokine-Stimulated Human Cortical SpheroidsBiomedicinesApril 29, 2022De Kleijn KMA, Straasheijm KR, Zuure WA, Martens GJM.flow of cytokines during the stimulation period. hCSs were incubated with 5 ng/mL TNFα (Sigma-Aldrich T0157-10UG; in PBS) or 5 ng/mL IL-1β (Sigma-Aldrich SRP3083-10UG; in PBS) for 4 h, 12 h or 36 h. The cytokine

(8) Myocardial Cytoskeletal Adaptations in Advanced Kidney DiseaseJournal of the American Heart AssociationMarch 1, 2022Arvin Halim, Gayatri Narayanan, Takashi Hato, Lilun Ho, Douglas Wan, Andrew M. Siedlecki, Eugene P. Rhee, Andrew S. Allegretti, Sagar U. Nigwekar, Daniel Zehnder, et al.uremic serum collected from end‐stage renal disease prior to hemodialysis, TNF‐α at 20 ng/mL (Cat. No. T0157; Sigma Aldrich, St Louis, MO) or mineral stress (DMEM supplemented with β‐glycerophosphate [Cat. No.

(9) Plasmodium falciparum Parasite Lines Expressing DC8 and Group A PfEMP1 Bind to Brain, Intestinal, and Kidney Endothelial CellsFrontiers in Cellular and Infection MicrobiologyJanuary 28, 2022Luana S. Ortolan, Marion Avril, Jun Xue, Karl B. Seydel, Ying Zheng, Joseph D. Smithproinflammatory pre-stimulation, confluent cell monolayers were stimulated with 10 ng/ml TNF-α (Sigma, T0157) for 20-24 hours at 37°C. Cells were analyzed in a LSRII (Becton & Dickson) with 100,000 events/sample.

(10) TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIVNature communicationsJanuary 10, 2022Matkovic R, Morel M, Lanciano S, Larrous P, Martin B, Bejjani F, Vauthier V, Hansen MMK, Emiliani S, Cristofari G, et al.latently infected with HIV-1 LTR-EGFP for 24 h. Cells were treated with 1 ng/ml TNFa (Sigma, catalog T0157) 16 h before fixation. The cells were washed with 10 mL of PBS solution and then immobilized on Cell-Tak

(11) Sox4 represses host innate immunity to facilitate pathogen infection by hijacking the TLR signaling networksVirulenceDecember 31, 2021Jian Shang et al.designed and synthesized by RiboBio (Guangzhou, China) and tested in a previous report [37]. TNF-α (T0157), CHX (N11534), BAY11-7082 (B5556), and the proteasome inhibitor MG-132 (M7449) were purchased from

(12) Exploiting polypharmacology to dissect host kinases and kinase inhibitors that modulate endothelial barrier integrityCell Chemical BiologyDecember 1, 2021Selasi Dankwa, Mary-Margaret Dols, Ling Wei, Elizabeth K.K. Glennon, Heather S. Kain, Alexis Kaushansky, Joseph D. Smith+ kinase inhibitor screen, except that HBMECs were activated with 10 ng/ml TNFα (Sigma-Aldrich Cat# T0157) for ~22 h before media equilibration on the day of the assay. Cells exposed to media only were set

(13) SARS-CoV-2 Spike Protein S1-Mediated Endothelial Injury and Pro-Inflammatory State Is Amplified by Dihydrotestosterone and Prevented by Mineralocorticoid AntagonismVirusesNovember 3, 2021Kumar N, Zuo Y, Yalavarthi S, Hunker KL, Knight JS, Kanthi Y, Obi AT, Ganesh SK.endothelial cells. TNF-α and valsartan were purchased from Sigma-Aldrich, St. Louis, MO, USA (Catalog: T0157) and Fisher Scientific, Waltham, MA, USA (Catalog: V01121G), respectively. 2.5. qRT-PCR At the end

(14) Myoinositol Reduces Inflammation and Oxidative Stress in Human Endothelial Cells Exposed In Vivo to Chronic HyperglycemiaNutrientsJune 27, 2021Maria Pompea Antonia Baldassarre, Pamela Di Tomo, Giorgia Centorame, Assunta Pandolfi, Natalia Di Pietro, Agostino Consoli, Gloria FormosoCAT. D8662), hydrogen peroxide (H2 O2 , CAT. H1009), and tumor necrosis factor-α human (TNF-α, CAT. T0157) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Fetal bovine serum (FBS, CAT. 41A0045K) and CellROX

(15) Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluationScientific reportsJune 23, 2021Slaughter VL, Rumsey JW, Boone R, Malik D, Cai Y, Sriram NN, Long CJ, McAleer CW, Lambert S, Shuler ML, et al.condition was mimicked by supplementing healthy BMM with 10 μM tumor necrosis factor alpha (TNF-α) (Sigma, T0157)45. The obese plasma condition was mimicked by supplementing healthy BMM with physiologically-relevant

(16) In vitro angiogenesis inhibition with selective compounds targeting the key glycolytic enzyme PFKFB3Pharmacological researchJune 1, 2021Abdali A, Baci D, Damiani I, Belloni F, De Dominicis C, Gelmi ML, Corsini A, Bellosta S.PA-1 and PA-2 in culture medium for 24 hours. After an hour incubation, human TNF-α (10 ng/mL; Sigma, T0157) was added to induce inflammation. For the preparation of total cell lysates, cells were washed in cold

(17) Targeted degradation of transcription factors by TRAFTACs: TRAnscription Factor TArgeting ChimerasCell Chemical BiologyMay 1, 2021Kusal T. G. Samarasinghe, Saul Jaime-Figueroa, Michael Burgess, Dhanusha A. Nalawansha, Katherine Dai, Zhenyi Hu, Adrian Bebenek, Scott A. Holley, Craig M. Crewsproteins","Chemicals, peptides, and recombinant proteins" Tumor Necrosis Factor-α human recombinant protein,Sigma,Cat# T0157–10UG dCas9 recombinant protein,Crews Lab,N/A Ni-NTA agarose,QIAGEN,Cat# 30250 Cycloheximide,Sigma,Cat#

(18) Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivityJournal of extracellular vesiclesApril 1, 2021Oggero S, de Gaetano M, Marcone S, Fitzsimons S, Pinto AL, Ikramova D, Barry M, Burke D, Montero-Melendez T, Cooper D, et al.re‐suspended in RPMI for further use. Blood aliquots (100 μl) were stimulated with TNF‐α (50 ng/ml; T0157‐10UG, Sigma‐Aldrich) or vehicle (PBS) for 1 h at 37 °C. After stimulation, erythrocytes were lysed with

(19) Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage deathThe Journal of experimental medicineFebruary 1, 2021Zhang L, Jiang X, Pfau D, Ling Y, Nathan CF.Plasmids with gRNA insertions were verified by Sanger sequencing. Mouse IFN-γ (#11276905001), TNFα (#T0157), CHX (#C4859), Rif (#R3501), Nec-1 (#N9037), Erastin (#E7781), RSL3 (#SML2234), and puromycin (#P8833)

(20) Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosisParticle and fibre toxicologyFebruary 1, 2021Annangi B, Lu Z, Bruniaux J, Ridoux A, da Silva VM, Vantelon D, Boczkowski J, Lanone S.peritoneal macrophages were obtained as previously described [57]. Briefly, 2 ml of sterile 4% thioglycolate broth (T0157, Sigma-Aldrich, La Verpillère, France) were administered in the peritoneal cavity of C57Bl/6 mice beforehand

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Tumor Necrosis Factor-α human from MilliporeSigma

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Tumor Necrosis Factor-α human from MilliporeSigma

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